|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CXCL7 ORF mammalian expression plasmid, C-GFPSpark tag||HG10878-ACG|
|Human CXCL7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10878-ACR|
|Human CXCL7 ORF mammalian expression plasmid, C-Flag tag||HG10878-CF|
|Human CXCL7 ORF mammalian expression plasmid, C-His tag||HG10878-CH|
|Human CXCL7 ORF mammalian expression plasmid, C-Myc tag||HG10878-CM|
|Human CXCL7 ORF mammalian expression plasmid, C-HA tag||HG10878-CY|
|Human CXCL7 Gene cDNA clone plasmid||HG10878-M|
|Human CXCL7 ORF mammalian expression plasmid, Flag tag||HG10878-M-F|
|Human CXCL7 ORF mammalian expression plasmid, N-Flag tag||HG10878-NF|
|Human CXCL7 ORF mammalian expression plasmid, N-His tag||HG10878-NH|
|Human CXCL7 ORF mammalian expression plasmid, N-Myc tag||HG10878-NM|
|Human CXCL7 ORF mammalian expression plasmid, N-HA tag||HG10878-NY|
|Human CXCL7 natural ORF mammalian expression plasmid||HG10878-UT|
|Learn more about expression Vectors|
Pro-platelet basic protein (PPBP) is also known as Chemokine (C-X-C motif) ligand 7 (CXCL7) and nucleosome assembly protein (Nap-2). Nap-2 / PPBP / CXCL7 is released in large amounts from platelets following their activation and is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. Nap-2 / PPBP / CXCL7 has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Nap-2 is a ligand for CXCR1 and CXCR2, and Nap-2, Nap-2 (73), Nap-2 (74), Nap-2 (1-66), and most potent Nap-2 (1-63) are chemoattractants and activators for neutrophils.