|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine TNFRSF9 ORF mammalian expression plasmid, C-GFPSpark tag||DG70095-ACG|
|Canine TNFRSF9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70095-ACR|
|Canine TNFRSF9 ORF mammalian expression plasmid, C-Flag tag||DG70095-CF|
|Canine TNFRSF9 ORF mammalian expression plasmid, C-His tag||DG70095-CH|
|Canine TNFRSF9 ORF mammalian expression plasmid, C-Myc tag||DG70095-CM|
|Canine TNFRSF9 ORF mammalian expression plasmid, C-HA tag||DG70095-CY|
|Canine TNFRSF9 Gene cDNA clone plasmid||DG70095-G|
|Canine TNFRSF9 ORF mammalian expression plasmid, N-Flag tag||DG70095-NF|
|Canine TNFRSF9 ORF mammalian expression plasmid, N-His tag||DG70095-NH|
|Canine TNFRSF9 ORF mammalian expression plasmid, N-Myc tag||DG70095-NM|
|Canine TNFRSF9 ORF mammalian expression plasmid, N-HA tag||DG70095-NY|
|Canine TNFRSF9 natural ORF mammalian expression plasmid||DG70095-UT|
|Learn more about expression Vectors|
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials.