|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human MFG-E8 ORF mammalian expression plasmid, C-GFPSpark tag||HG10853-ACG|
|Human MFG-E8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10853-ACR|
|Human MFG-E8 ORF mammalian expression plasmid, C-Flag tag||HG10853-CF|
|Human MFG-E8 ORF mammalian expression plasmid, C-His tag||HG10853-CH|
|Human MFG-E8 ORF mammalian expression plasmid, C-Myc tag||HG10853-CM|
|Human MFG-E8 ORF mammalian expression plasmid, C-HA tag||HG10853-CY|
|Human MFG-E8 Gene cDNA clone plasmid||HG10853-M|
|Human MFG-E8 ORF mammalian expression plasmid, His tag||HG10853-M-H|
|Human MFG-E8 ORF mammalian expression plasmid, N-Flag tag||HG10853-NF|
|Human MFG-E8 ORF mammalian expression plasmid, N-His tag||HG10853-NH|
|Human MFG-E8 ORF mammalian expression plasmid, N-Myc tag||HG10853-NM|
|Human MFG-E8 ORF mammalian expression plasmid, N-HA tag||HG10853-NY|
|Human MFG-E8 natural ORF mammalian expression plasmid||HG10853-UT|
|Learn more about expression Vectors|
MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.