|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine CX3CL1 ORF mammalian expression plasmid, C-GFPSpark tag||DG70071-ACG|
|Canine CX3CL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70071-ACR|
|Canine CX3CL1 ORF mammalian expression plasmid, C-Flag tag||DG70071-CF|
|Canine CX3CL1 ORF mammalian expression plasmid, C-His tag||DG70071-CH|
|Canine CX3CL1 ORF mammalian expression plasmid, C-Myc tag||DG70071-CM|
|Canine CX3CL1 ORF mammalian expression plasmid, C-HA tag||DG70071-CY|
|Canine CX3CL1 Gene cDNA clone plasmid||DG70071-G|
|Canine CX3CL1 ORF mammalian expression plasmid, N-Flag tag||DG70071-NF|
|Canine CX3CL1 ORF mammalian expression plasmid, N-His tag||DG70071-NH|
|Canine CX3CL1 ORF mammalian expression plasmid, N-Myc tag||DG70071-NM|
|Canine CX3CL1 ORF mammalian expression plasmid, N-HA tag||DG70071-NY|
|Canine CX3CL1 natural ORF mammalian expression plasmid||DG70071-UT|
|Learn more about expression Vectors|
Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.