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Bcl-XL / BCL2L1 Antibody, Rabbit PAb, Antigen Affinity Purified

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Mouse BCL2L1 Antibody Product Information
Immunogen:Recombinant Mouse Bcl-XL / BCL2L1 protein (Catalog#50012-M08E)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Mouse Bcl-XL / BCL2L1 (rM Bcl-XL / BCL2L1; Catalog#50012-M08E; Q64373-1; Met1-Arg212). Bcl-XL / BCL2L1 specific IgG was purified by Mouse Bcl-XL / BCL2L1 affinity chromatography.
Mouse BCL2L1 Antibody Usage Guide
Specificity:Mouse Bcl-XL / BCL2L1
Application:ELISA, IHC-P

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse Bcl-XL / BCL2L1. The detection limit for Mouse Bcl-XL / BCL2L1 is approximately 0.00975 ng/well.

IHC-P: 0.1-2 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Mouse BCL2L1 Antibody IHC Application Image
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Caption:
Immunochemical staining of mouse BCL2L1 in mouse brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Bcl-XL / BCL2L1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of mouse BCL2L1 in mouse kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nephric tubule.
Bcl-XL / BCL2L1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Other BCL2L1 Antibody Products
BCL2L1/Bcl-XL Background

B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1) , belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.

Mouse BCL2L1/Bcl-XL References
  • Vander Heiden MG, et al. (1997) Bcl-xL Regulates the Membrane Potential and Volume Homeostasis of Mitochondria. Cell. 91 (5): 627-37.
  • Muchmore SW, et al. (1996) X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature. 381: 335-341.
  • SharoffEH, et al. (2007) Bcl-2 family members regulate anoxia-induced cell death. Antioxid Redox Signal. 9 (9) :1405-9.
  • Zhou F, et al. (2011) Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis. FEBS J. 278 (3): 403-13.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"