|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CXADR ORF mammalian expression plasmid, C-GFPSpark tag||HG10799-ACG|
|Human CXADR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10799-ACR|
|Human CXADR ORF mammalian expression plasmid, C-Flag tag||HG10799-CF|
|Human CXADR ORF mammalian expression plasmid, C-His tag||HG10799-CH|
|Human CXADR ORF mammalian expression plasmid, C-Myc tag||HG10799-CM|
|Human CXADR ORF mammalian expression plasmid, C-HA tag||HG10799-CY|
|Human CXADR Gene cDNA clone plasmid||HG10799-M|
|Human CXADR ORF mammalian expression plasmid, N-Flag tag||HG10799-NF|
|Human CXADR ORF mammalian expression plasmid, N-His tag||HG10799-NH|
|Human CXADR ORF mammalian expression plasmid, N-Myc tag||HG10799-NM|
|Human CXADR ORF mammalian expression plasmid, N-HA tag||HG10799-NY|
|Human CXADR natural ORF mammalian expression plasmid||HG10799-UT|
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CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.