|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human BACE2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10783-ACG|
|Human BACE2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10783-ACR|
|Human BACE2 ORF mammalian expression plasmid, C-Flag tag||HG10783-CF|
|Human BACE2 ORF mammalian expression plasmid, C-His tag||HG10783-CH|
|Human BACE2 ORF mammalian expression plasmid, C-Myc tag||HG10783-CM|
|Human BACE2 ORF mammalian expression plasmid, C-HA tag||HG10783-CY|
|Human BACE2 Gene cDNA clone plasmid||HG10783-M|
|Human BACE2 ORF mammalian expression plasmid, N-Flag tag||HG10783-NF|
|Human BACE2 ORF mammalian expression plasmid, N-His tag||HG10783-NH|
|Human BACE2 ORF mammalian expression plasmid, N-Myc tag||HG10783-NM|
|Human BACE2 ORF mammalian expression plasmid, N-HA tag||HG10783-NY|
|Human BACE2 natural ORF mammalian expression plasmid||HG10783-UT|
|Learn more about expression Vectors|
BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It is a protease known to be an important enzyme involved in the cellular pathways. BACE2 has been shown to interact with GGA1 and GGA2. It is the major β-secretase in vivo. BACE2 is located on chromosome 21 and may play a role in alzheimer's disease pathogenesis in down syndrome(DS). Overexpression of BACE2 by lentivirus markedly reduced amyloid β protein production in primary neurons. Despite an extra copy of the BACE2 gene in DS and the increase of its transcription, BACE2 protein levels are unchanged.