|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TGFBR3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10778-ACG|
|Human TGFBR3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10778-ACR|
|Human TGFBR3 ORF mammalian expression plasmid, C-Flag tag||HG10778-CF|
|Human TGFBR3 ORF mammalian expression plasmid, C-His tag||HG10778-CH|
|Human TGFBR3 ORF mammalian expression plasmid, C-Myc tag||HG10778-CM|
|Human TGFBR3 ORF mammalian expression plasmid, C-HA tag||HG10778-CY|
|Human TGFBR3 Gene cDNA clone plasmid||HG10778-M|
|Human TGFBR3 natural ORF mammalian expression plasmid||HG10778-M-N|
|Human TGFBR3 ORF mammalian expression plasmid, N-Flag tag||HG10778-NF|
|Human TGFBR3 ORF mammalian expression plasmid, N-His tag||HG10778-NH|
|Human TGFBR3 ORF mammalian expression plasmid, N-Myc tag||HG10778-NM|
|Human TGFBR3 ORF mammalian expression plasmid, N-HA tag||HG10778-NY|
|Human TGFBR3 natural ORF mammalian expression plasmid||HG10778-UT|
|Learn more about expression Vectors|
Betaglycan also known as transforming growth factor beta receptor III (TGFBR3), is a cell-surface chondroitin sulfate / heparan sulfate proteoglycan. TGFBR3 is a transforming growth factor (TGF)-beta type III receptor. This receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. TGFBR3 is the TGF-β component most commonly downregulated among localized human prostate cancer studies. TGFBR3 knockdown led to focus formation and enhanced expression of CD133, a marker found on prostate cancer stem cells. TGFBR3 is an accessory receptor that binds to and modulates the activities of both transforming growth factor-beta (TGFβ) and inhibin, two members of the TGFβ superfamily of growth factors that regulate many aspects of reproductive biology. TGFBR3 is known to be expressed in adult testis and ovary, but little is known about this receptor during gonadogenesis.