|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human Acrp30 ORF mammalian expression plasmid, C-GFPSpark tag||HG10772-ACG|
|Human Acrp30 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10772-ACR|
|Human Acrp30 ORF mammalian expression plasmid, C-Flag tag||HG10772-CF|
|Human Acrp30 ORF mammalian expression plasmid, C-His tag||HG10772-CH|
|Human Acrp30 ORF mammalian expression plasmid, C-Myc tag||HG10772-CM|
|Human Acrp30 ORF mammalian expression plasmid, C-HA tag||HG10772-CY|
|Human Acrp30 Gene cDNA clone plasmid||HG10772-M|
|Human Acrp30 natural ORF mammalian expression plasmid||HG10772-M-N|
|Human Acrp30 ORF mammalian expression plasmid, N-Flag tag||HG10772-NF|
|Human Acrp30 ORF mammalian expression plasmid, N-His tag||HG10772-NH|
|Human Acrp30 ORF mammalian expression plasmid, N-Myc tag||HG10772-NM|
|Human Acrp30 ORF mammalian expression plasmid, N-HA tag||HG10772-NY|
|Human Acrp30 natural ORF mammalian expression plasmid||HG10772-UT|
|Learn more about expression Vectors|
Adiponectin (ADIPOQ), or 30 kDa adipocyte complement-related protein (Acrp30) is a protein secreted by adipose tissue, which acts to reduce insulin resistance and atherogenic damage, but it also exerts actions in other tissues. Adiponectin mediates its actions in the periphery mainly via two receptors, AdipoR1 and AdipoR2. Adiponectin influences gonadotropin release, normal pregnancy, and assisted reproduction outcomes. Adiponectin, a beneficial adipokine, represents a major link between obesity and reproduction. Higher levels of adiponectin are associated with improved menstrual function and better outcomes in assisted reproductive cycles. Unlike other adipocytokines produced by adipose tissue, adiponectin appears to have anti-inflammatory, anti-diabetic, and anti-atherogenic properties. Several clinical studies demonstrate the inverse relationship between plasma adiponectin levels and several inflammatory markers including C-reactive protein. Adiponectin attenuates inflammatory responses to multiple stimuli by modulating signaling pathways in a variety of cell types. The anti-inflammatory properties of adiponectin may be a major component of its beneficial effects on cardiovascular and metabolic disorders including atherosclerosis and insulin resistance. Additionally, it is important factor in chronic liver diseases and chronic kidney diseases. Some cancer cell types express adiponectin receptors. Thus Adiponectin may act on tumour cells directly by binding and activating adiponectin receptors and downstream signalling pathways.