|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human AKT1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10763-ACG|
|Human AKT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10763-ACR|
|Human AKT1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10763-ANG|
|Human AKT1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10763-ANR|
|Human AKT1 ORF mammalian expression plasmid, C-Flag tag||HG10763-CF|
|Human AKT1 ORF mammalian expression plasmid, C-His tag||HG10763-CH|
|Human AKT1 ORF mammalian expression plasmid, C-Myc tag||HG10763-CM|
|Human AKT1 ORF mammalian expression plasmid, C-HA tag||HG10763-CY|
|Human AKT1 Gene cDNA clone plasmid||HG10763-G|
|Human AKT1 ORF mammalian expression plasmid, N-Flag tag||HG10763-NF|
|Human AKT1 ORF mammalian expression plasmid, N-His tag||HG10763-NH|
|Human AKT1 ORF mammalian expression plasmid, N-Myc tag||HG10763-NM|
|Human AKT1 ORF mammalian expression plasmid, N-HA tag||HG10763-NY|
|Human AKT1 natural ORF mammalian expression plasmid||HG10763-UT|
|Learn more about expression Vectors|
v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis.