|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SEMA3A ORF mammalian expression plasmid, C-GFPSpark tag||HG10758-ACG|
|Human SEMA3A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10758-ACR|
|Human SEMA3A ORF mammalian expression plasmid, C-Flag tag||HG10758-CF|
|Human SEMA3A ORF mammalian expression plasmid, C-His tag||HG10758-CH|
|Human SEMA3A ORF mammalian expression plasmid, C-Myc tag||HG10758-CM|
|Human SEMA3A ORF mammalian expression plasmid, C-HA tag||HG10758-CY|
|Human SEMA3A Gene cDNA clone plasmid||HG10758-M|
|Human SEMA3A natural ORF mammalian expression plasmid||HG10758-M-N|
|Human SEMA3A ORF mammalian expression plasmid, N-Flag tag||HG10758-NF|
|Human SEMA3A ORF mammalian expression plasmid, N-His tag||HG10758-NH|
|Human SEMA3A ORF mammalian expression plasmid, N-Myc tag||HG10758-NM|
|Human SEMA3A ORF mammalian expression plasmid, N-HA tag||HG10758-NY|
|Human SEMA3A natural ORF mammalian expression plasmid||HG10758-UT|
|Learn more about expression Vectors|
Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer's disease