|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human GP1BB ORF mammalian expression plasmid, C-GFPSpark tag||HG10742-ACG|
|Human GP1BB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10742-ACR|
|Human GP1BB ORF mammalian expression plasmid, C-Flag tag||HG10742-CF|
|Human GP1BB ORF mammalian expression plasmid, C-His tag||HG10742-CH|
|Human GP1BB ORF mammalian expression plasmid, C-Myc tag||HG10742-CM|
|Human GP1BB ORF mammalian expression plasmid, C-HA tag||HG10742-CY|
|Human GP1BB Gene cDNA clone plasmid||HG10742-M|
|Human GP1BB ORF mammalian expression plasmid, Flag tag||HG10742-M-F|
|Human GP1BB ORF mammalian expression plasmid, N-Flag tag||HG10742-NF|
|Human GP1BB ORF mammalian expression plasmid, N-His tag||HG10742-NH|
|Human GP1BB ORF mammalian expression plasmid, N-Myc tag||HG10742-NM|
|Human GP1BB ORF mammalian expression plasmid, N-HA tag||HG10742-NY|
|Human GP1BB natural ORF mammalian expression plasmid||HG10742-UT|
|Learn more about expression Vectors|
Platelet glycoprotein Ib (GPIb) complex is best known as a major platelet receptor for von Willebrand factor essential for platelet adhesion under high shear conditions found in arteries and in thrombosis. The GPIb complex is composed of GPIb alpha (Platelet glycoprotein Ib alpha chain) covalently attached to GPIb beta (Platelet glycoprotein Ib beta chain) and noncovalently complexed with GPIX and GPV. GPIb-beta, also known as GP1BB, CD42b-beta and CD42c, is single-pass type I membrane protein expressed in heart and brain, which is a critical component of the von Willebrand factor (vWF) receptor. The cysteine knot region of GPIb beta in the N terminus is critical for the conformation of GPIb beta that interacts with GPIX. The precursor of GP1BB is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. GPIb is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. GPIb alpha chain provides the vWF binding site, and GPIb beta chain contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. GP1BB is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (vWF), and mediates platelet adhesion in the arterial circulation. Defects in GP1BB are a cause of Bernard-Soulier syndrome (BSS), also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.