|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CSK ORF mammalian expression plasmid, C-GFPSpark tag||HG10740-ACG|
|Human CSK ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10740-ACR|
|Human CSK ORF mammalian expression plasmid, N-GFPSpark tag||HG10740-ANG|
|Human CSK ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10740-ANR|
|Human CSK ORF mammalian expression plasmid, C-Flag tag||HG10740-CF|
|Human CSK ORF mammalian expression plasmid, C-His tag||HG10740-CH|
|Human CSK ORF mammalian expression plasmid, C-Myc tag||HG10740-CM|
|Human CSK ORF mammalian expression plasmid, C-HA tag||HG10740-CY|
|Human CSK Gene cDNA clone plasmid||HG10740-M|
|Human CSK ORF mammalian expression plasmid, N-Flag tag||HG10740-NF|
|Human CSK ORF mammalian expression plasmid, N-His tag||HG10740-NH|
|Human CSK ORF mammalian expression plasmid, N-Myc tag||HG10740-NM|
|Human CSK ORF mammalian expression plasmid, N-HA tag||HG10740-NY|
|Human CSK natural ORF mammalian expression plasmid||HG10740-UT|
|Learn more about expression Vectors|
The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development.