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Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-tagged

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TRIB2cDNA Clone Product Information
Gene_bank_ref_id:NM_021643.3
cDNA Size:1032
cDNA Description:ORF Clone of Homo sapiens tribbles homolog 2 (Drosophila) DNA.
Gene Synonym:C5FW, TRB2, GS3955, TRIB2
Species:Human
Vector:pCMV3-C-Myc
Restriction Site:
Tag Sequence:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-C-Myc Vector Information
 
Vector Name pCMV3-C-Myc
Vector Size 6164bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag Myc
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-C-Myc Physical Map

Schematic of pCMV3-C-Myc Multiple Cloning Sites

Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-tagged on other vectors
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10725-ACG$325
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10725-ACR$325
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10725-ANG$325
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10725-ANR$325
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10725-CF$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10725-CH$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10725-CM$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10725-CY$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone)HG10725-M$95
Human TRIB2 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10725-M-F$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10725-M-N$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10725-NF$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10725-NH$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10725-NM$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10725-NY$295
Human TRIB2 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10725-UT$295
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Background

Tribbles homolog 2, also known as TRB-2, and Trib2, is a member of the protein kinase superfamily and Tribbles subfamily (Trib1, Trib2, Trib3). The identification of tribbles as regulators of signal processing systems and physiological processes, including development, together with their potential involvement in diabetes and cancer, has generated considerable interest in these proteins. Tribbles have been reported to regulate activation of a number of intracellular signalling pathways with roles extending from mitosis and cell activation to apoptosis and modulation of gene expression. Tribbles controls the timing of mitosis in the prospective mesoderm, allowing cell-shape changes to be completed. This mechanism for coordinating cell division and cell-shape changes may have helped Drosophila to evolve its mode of rapid early development. Trib2 was identified as a downregulated transcript in leukemic cells undergoing growth arrest. Trib2-transduced bone marrow cells exhibited a growth advantage and readily established factor-dependent cell lines. Trib2-reconstituted mice uniformly developed fatal transplantable acute myelogenous leukemia (AML).

References
  • Seher, TC. et al., 2000, Curr Biol. 10 (11): 623-9.
  • Keeshan, K. et al., 2006, Cancer Cell. 10 (5): 401-11.
  • Hegedus, Z. et al., 2007, Cell Signal. 19 (2):238-50.
  • Keeshan, K. et al., 2008, Blood Cells Mol Dis. 40 (1): 119-21.
  • Cvetkovic, LV. et al., 2010, J Clin Invest. 120 (3): 713-9.
  • Size / Price
    Catalog:HG10725-CM
    List Price: $295.00  (Save $0.00)
    Price:$295.00      [How to order]
    Availability2-3 weeks
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