|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human sFRP-4 ORF mammalian expression plasmid, C-GFPSpark tag||HG10717-ACG|
|Human sFRP-4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10717-ACR|
|Human sFRP-4 ORF mammalian expression plasmid, C-Flag tag||HG10717-CF|
|Human sFRP-4 ORF mammalian expression plasmid, C-His tag||HG10717-CH|
|Human sFRP-4 ORF mammalian expression plasmid, C-Myc tag||HG10717-CM|
|Human sFRP-4 ORF mammalian expression plasmid, C-HA tag||HG10717-CY|
|Human sFRP-4 ORF mammalian expression plasmid, N-Flag tag||HG10717-NF|
|Human sFRP-4 ORF mammalian expression plasmid, N-His tag||HG10717-NH|
|Human sFRP-4 ORF mammalian expression plasmid, N-Myc tag||HG10717-NM|
|Human sFRP-4 ORF mammalian expression plasmid, N-HA tag||HG10717-NY|
|Human sFRP-4 Gene cDNA clone plasmid||HG10717-U|
|Human sFRP-4 ORF mammalian expression plasmid, His tag||HG10717-U-H|
|Human sFRP-4 natural ORF mammalian expression plasmid||HG10717-UT|
|Learn more about expression Vectors|
SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. SFRP4 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain . Mouse SFRP4 is highly expressed in the ovary, and is localized to granulosa cells of periovulatory follicles and corpora lutea. It plays a critical role in placental development and implantation, and is also an important factor in the development of the decidual fibrinoid zone, and in trophoblast apoptosis.