|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PLK1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10676-ACG|
|Human PLK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10676-ACR|
|Human PLK1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10676-ANG|
|Human PLK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10676-ANR|
|Human PLK1 ORF mammalian expression plasmid, C-Flag tag||HG10676-CF|
|Human PLK1 ORF mammalian expression plasmid, C-His tag||HG10676-CH|
|Human PLK1 ORF mammalian expression plasmid, C-Myc tag||HG10676-CM|
|Human PLK1 ORF mammalian expression plasmid, C-HA tag||HG10676-CY|
|Human PLK1 Gene cDNA clone plasmid||HG10676-M|
|Human PLK1 ORF mammalian expression plasmid, Flag tag||HG10676-M-F|
|Human PLK1 ORF mammalian expression plasmid, N-Flag tag||HG10676-NF|
|Human PLK1 ORF mammalian expression plasmid, N-His tag||HG10676-NH|
|Human PLK1 ORF mammalian expression plasmid, N-Myc tag||HG10676-NM|
|Human PLK1 ORF mammalian expression plasmid, N-HA tag||HG10676-NY|
|Human PLK1 natural ORF mammalian expression plasmid||HG10676-UT|
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Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.