|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human Syndecan-4 ORF mammalian expression plasmid, C-GFPSpark tag||HG10653-ACG|
|Human Syndecan-4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10653-ACR|
|Human Syndecan-4 ORF mammalian expression plasmid, C-Flag tag||HG10653-CF|
|Human Syndecan-4 ORF mammalian expression plasmid, C-His tag||HG10653-CH|
|Human Syndecan-4 ORF mammalian expression plasmid, C-Myc tag||HG10653-CM|
|Human Syndecan-4 ORF mammalian expression plasmid, C-HA tag||HG10653-CY|
|Human Syndecan-4 Gene cDNA clone plasmid||HG10653-M|
|Human Syndecan-4 ORF mammalian expression plasmid, N-Flag tag||HG10653-NF|
|Human Syndecan-4 ORF mammalian expression plasmid, N-His tag||HG10653-NH|
|Human Syndecan-4 ORF mammalian expression plasmid, N-Myc tag||HG10653-NM|
|Human Syndecan-4 ORF mammalian expression plasmid, N-HA tag||HG10653-NY|
|Human Syndecan-4 natural ORF mammalian expression plasmid||HG10653-UT|
|Learn more about expression Vectors|
SDC4 (Syndecan-4), also known as Syn4, is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. Syndecan-4 plays roles in the formation of focal adhesions and stress fibers. The cytoplasmic domain of syndecan-4 interacts with a number of signalling and structural proteins, and both extracellular and cytoplasmic domains are necessary for regulated activation of associated transmembrane receptors. Syndecan-4/SDC4 is a heparan sulfate proteoglycan and works as a coreceptor for various growth factors. SDC4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cells (VSMCs) proliferation and vascular progenitor cells (VPCs) mobilization. Therefore, SDC4 may be a novel therapeutic target for preventing arterial restenosis after angioplasty.