|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TNFSF13 ORF mammalian expression plasmid, C-GFPSpark tag||HG10610-ACG|
|Human TNFSF13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10610-ACR|
|Human TNFSF13 ORF mammalian expression plasmid, C-Flag tag||HG10610-CF|
|Human TNFSF13 ORF mammalian expression plasmid, C-His tag||HG10610-CH|
|Human TNFSF13 ORF mammalian expression plasmid, C-Myc tag||HG10610-CM|
|Human TNFSF13 ORF mammalian expression plasmid, C-HA tag||HG10610-CY|
|Human TNFSF13 Gene cDNA clone plasmid||HG10610-M|
|Human TNFSF13 ORF mammalian expression plasmid, N-Flag tag||HG10610-NF|
|Human TNFSF13 ORF mammalian expression plasmid, N-His tag||HG10610-NH|
|Human TNFSF13 ORF mammalian expression plasmid, N-Myc tag||HG10610-NM|
|Human TNFSF13 ORF mammalian expression plasmid, N-HA tag||HG10610-NY|
|Human TNFSF13 natural ORF mammalian expression plasmid||HG10610-UT|
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TNFSF13 is a member of the tumor necrosis factor (TNF) ligand family. It is a ligand for TNFRSF17/BCMA. TNFSF13 is lowly expressed in normal tissues, but is elevated in several types of tumors and transformed cell lines. It is mportant for B cell development. TNFSF13 may also play a role in T-independent type II antigen responses and T cell survival, and induce proliferation/survival of non lymphoid cells. It exists as a functional homotrimer. It can bind to two cell surface receptors, BCMA and TACI, which it shares with BAFF to exert downstream T- and B-cell regulatory effects. TNFSF13 also has been demonstrated to bind to proteoglycans on the cell surface.