|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human HGF ORF mammalian expression plasmid, C-GFPSpark tag||HG10463-ACG|
|Human HGF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10463-ACR|
|Human HGF ORF mammalian expression plasmid, C-Flag tag||HG10463-CF|
|Human HGF ORF mammalian expression plasmid, C-His tag||HG10463-CH|
|Human HGF ORF mammalian expression plasmid, C-Myc tag||HG10463-CM|
|Human HGF ORF mammalian expression plasmid, C-HA tag||HG10463-CY|
|Human HGF Gene cDNA clone plasmid||HG10463-M|
|Human HGF ORF mammalian expression plasmid, N-Flag tag||HG10463-NF|
|Human HGF ORF mammalian expression plasmid, N-His tag||HG10463-NH|
|Human HGF ORF mammalian expression plasmid, N-Myc tag||HG10463-NM|
|Human HGF ORF mammalian expression plasmid, N-HA tag||HG10463-NY|
|Human HGF natural ORF mammalian expression plasmid||HG10463-UT|
|Learn more about expression Vectors|
Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.