|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TGFBR1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10459-ACG|
|Human TGFBR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10459-ACR|
|Human TGFBR1 ORF mammalian expression plasmid, C-Flag tag||HG10459-CF|
|Human TGFBR1 ORF mammalian expression plasmid, C-His tag||HG10459-CH|
|Human TGFBR1 ORF mammalian expression plasmid, C-Myc tag||HG10459-CM|
|Human TGFBR1 ORF mammalian expression plasmid, C-HA tag||HG10459-CY|
|Human TGFBR1 Gene cDNA clone plasmid||HG10459-M|
|Human TGFBR1 ORF mammalian expression plasmid, Flag tag||HG10459-M-F|
|Human TGFBR1 ORF mammalian expression plasmid, N-Flag tag||HG10459-NF|
|Human TGFBR1 ORF mammalian expression plasmid, N-His tag||HG10459-NH|
|Human TGFBR1 ORF mammalian expression plasmid, N-Myc tag||HG10459-NM|
|Human TGFBR1 ORF mammalian expression plasmid, N-HA tag||HG10459-NY|
|Human TGFBR1 natural ORF mammalian expression plasmid||HG10459-UT|
|Learn more about expression Vectors|
Transforming growth factor, beta receptor I, also known as Transforming growth factor-beta receptor type I , Serine / threonine-protein kinase receptor R4, Activin receptor-like kinase 5, SKR4, ALK-5, and TGFBR1, is a single-pass type I membrane protein which belongs to the protein kinase superfamily and TGFB receptor subfamily. TGFBR1 / ALK-5 is found in all tissues examined. It is most abundant in placenta and least abundant in brain and heart. TGF-beta functions as a tumor suppressor by inhibiting the cell cycle in the G1 phase. Administration of TGF-beta is able to protect against mammary tumor development in transgenic mouse models in vivo. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers, with the majority of colon and gastric cancers being caused by an inactivating mutation of TGF-beta RII. On ligand binding, TGFBR1 / ALK-5 forms a receptor complex consisting of two type I I and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which auto-phosphorylate, then bind and activate SMAD transcriptional regulators. TGF-beta signaling via TGFBR1 / ALK-5 is not required in myocardial cells during mammalian cardiac development, but plays an irreplaceable cell-autonomous role regulating cellular communication, differentiation and proliferation in endocardial and epicardial cells. Defects in TGFBR1 / ALK-5 are the cause of Loeys-Dietz syndrome type 1A (LDS1A), Loeys-Dietz syndrome type 2A (LDS2A), and aortic aneurysm familial thoracic type 5 (AAT5).