|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human WISP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10442-ACG|
|Human WISP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10442-ACR|
|Human WISP1 ORF mammalian expression plasmid, C-Flag tag||HG10442-CF|
|Human WISP1 ORF mammalian expression plasmid, C-His tag||HG10442-CH|
|Human WISP1 ORF mammalian expression plasmid, C-Myc tag||HG10442-CM|
|Human WISP1 ORF mammalian expression plasmid, C-HA tag||HG10442-CY|
|Human WISP1 Gene cDNA clone plasmid||HG10442-M|
|Human WISP1 ORF mammalian expression plasmid, N-Flag tag||HG10442-NF|
|Human WISP1 ORF mammalian expression plasmid, N-His tag||HG10442-NH|
|Human WISP1 ORF mammalian expression plasmid, N-Myc tag||HG10442-NM|
|Human WISP1 ORF mammalian expression plasmid, N-HA tag||HG10442-NY|
|Human WISP1 natural ORF mammalian expression plasmid||HG10442-UT|
|Learn more about expression Vectors|
CCN4/Wnt-induced secreted protein 1 (WISP1) is a secreted, cysteine-rich, heparin-binding glycoprotein, belonging to the CCN (CTGF/CYR61/NOV) family of growth factors, and is involved in diverse biological functions such as cell growth, adhesion, migration, angiogenesis, tissue repair, and regulation of extracellular matrix. Members of the CCN family demonstrate high structural homology sharing four conserved cysteine-rich modular domains: a IGFBP (insulin-like growthfactor-binding) domain, a von Willebrand type C domain, a thrombospondin domain and a C-terminal cysteine -knot domain. WISP1 is a putative downstream effector of the Wnt/Frizzled pathway that mediates diversedevelopmental processes, was identified as an oncogene regulated by the Wnt-1-beta-catenin pathway. Thus may contributes to Wnt-1-mediated tumorigenesis and malignance. Expression of WISP1 in some cells results in transformation and tumorigenesis. WISP1 acts to block cell death at a late stage in the p53-mediated apoptosis pathway. It was reported that WISP1 interacts with sulfated glycoconjugates, decorin and biglycan in the ECM of connective tissue, and possibly prevents their inhibitory activity in tumor cell proliferation.