|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human Vitronectin ORF mammalian expression plasmid, C-GFPSpark tag||HG10424-ACG|
|Human Vitronectin ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10424-ACR|
|Human Vitronectin ORF mammalian expression plasmid, C-Flag tag||HG10424-CF|
|Human Vitronectin ORF mammalian expression plasmid, C-His tag||HG10424-CH|
|Human Vitronectin ORF mammalian expression plasmid, C-Myc tag||HG10424-CM|
|Human Vitronectin ORF mammalian expression plasmid, C-HA tag||HG10424-CY|
|Human Vitronectin Gene cDNA clone plasmid||HG10424-G|
|Human Vitronectin ORF mammalian expression plasmid, N-Flag tag||HG10424-NF|
|Human Vitronectin ORF mammalian expression plasmid, N-His tag||HG10424-NH|
|Human Vitronectin ORF mammalian expression plasmid, N-Myc tag||HG10424-NM|
|Human Vitronectin ORF mammalian expression plasmid, N-HA tag||HG10424-NY|
|Human Vitronectin natural ORF mammalian expression plasmid||HG10424-UT|
|Learn more about expression Vectors|
Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin is a plasma glycoprotein implicated as a regulator of diverse physiological process, including blood coagulation, fibrinolysis, pericellular proteolysis, complement dependent immune responses, and cell attachment and spreading. Because of its ability to bind platelet glycoproteins and mediate platelet adhesion and aggregation at sites of vascular injury, vitronectin has become an important mediator in the pathogenesis of coronary atherosclerosis. As a multifunctional protein with a multiple binding domain, Vitronectin interacts with a variety of plasma and cell proteins. Vitronectin binds multiple ligands, including the soluble vitronectin receptor. It may be an independent predictor of adverse cardiovascular outcomes following acute stenting. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.