|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TIMP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10396-ACG|
|Human TIMP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10396-ACR|
|Human TIMP2 ORF mammalian expression plasmid, C-Flag tag||HG10396-CF|
|Human TIMP2 ORF mammalian expression plasmid, C-His tag||HG10396-CH|
|Human TIMP2 ORF mammalian expression plasmid, C-Myc tag||HG10396-CM|
|Human TIMP2 ORF mammalian expression plasmid, C-HA tag||HG10396-CY|
|Human TIMP2 Gene cDNA clone plasmid||HG10396-M|
|Human TIMP2 ORF mammalian expression plasmid, Flag tag||HG10396-M-F|
|Human TIMP2 ORF mammalian expression plasmid, N-Flag tag||HG10396-NF|
|Human TIMP2 ORF mammalian expression plasmid, N-His tag||HG10396-NH|
|Human TIMP2 ORF mammalian expression plasmid, N-Myc tag||HG10396-NM|
|Human TIMP2 ORF mammalian expression plasmid, N-HA tag||HG10396-NY|
|Human TIMP2 natural ORF mammalian expression plasmid||HG10396-UT|
|Learn more about expression Vectors|
Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and they inhibit most MMPs by forming non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated protein expressed by a variety of cell types, and plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors. Findings establish an unexpected, MMP-independent mechanism for TIMP2 inhibition of endothelial cell proliferation in vitro and reveal an important component of the antiangiogenic effect of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance of tissue homeostasis and is involved in the regulation of tumor microenvironment.