|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human IFNαG ORF mammalian expression plasmid, C-GFPSpark tag||HG10342-ACG|
|Human IFNαG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10342-ACR|
|Human IFNαG ORF mammalian expression plasmid, C-Flag tag||HG10342-CF|
|Human IFNαG ORF mammalian expression plasmid, C-His tag||HG10342-CH|
|Human IFNαG ORF mammalian expression plasmid, C-Myc tag||HG10342-CM|
|Human IFNαG ORF mammalian expression plasmid, C-HA tag||HG10342-CY|
|Human IFNαG Gene cDNA clone plasmid||HG10342-M|
|Human IFNαG ORF mammalian expression plasmid, Flag tag||HG10342-M-F|
|Human IFNαG ORF mammalian expression plasmid, N-Flag tag||HG10342-NF|
|Human IFNαG ORF mammalian expression plasmid, N-His tag||HG10342-NH|
|Human IFNαG ORF mammalian expression plasmid, N-Myc tag||HG10342-NM|
|Human IFNαG ORF mammalian expression plasmid, N-HA tag||HG10342-NY|
|Human IFNαG natural ORF mammalian expression plasmid||HG10342-UT|
|Learn more about expression Vectors|
Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.