|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human Cadherin-12 ORF mammalian expression plasmid, C-GFPSpark tag||HG10317-ACG|
|Human Cadherin-12 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10317-ACR|
|Human Cadherin-12 ORF mammalian expression plasmid, C-Flag tag||HG10317-CF|
|Human Cadherin-12 ORF mammalian expression plasmid, C-His tag||HG10317-CH|
|Human Cadherin-12 ORF mammalian expression plasmid, C-Myc tag||HG10317-CM|
|Human Cadherin-12 ORF mammalian expression plasmid, C-HA tag||HG10317-CY|
|Human Cadherin-12 Gene cDNA clone plasmid||HG10317-M|
|Human Cadherin-12 ORF mammalian expression plasmid, N-Flag tag||HG10317-NF|
|Human Cadherin-12 ORF mammalian expression plasmid, N-His tag||HG10317-NH|
|Human Cadherin-12 ORF mammalian expression plasmid, N-Myc tag||HG10317-NM|
|Human Cadherin-12 ORF mammalian expression plasmid, N-HA tag||HG10317-NY|
|Human Cadherin-12 natural ORF mammalian expression plasmid||HG10317-UT|
|Learn more about expression Vectors|
Classic Cadherins represent a family of calcium-dependent homophilic cell-cell adhesion molecules. They confer strong adhesiveness to animal cells when they are anchored to the actin cytoskeleton via their cytoplasmic binding partners, catenins. The cadherin/catenin adhesion system plays key roles in the morphogenesis and function of the vertebrate and invertebrate nervous systems. Furthermore, this system is involved in synaptic plasticity. Recent studies on the role of individual cadherin subtypes at synapses indicate that individual cadherin subtypes play their own unique role to regulate synaptic activities. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-12 also known as CDH12, is a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherin-12 appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.