|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human MERTK / Mer ORF mammalian expression plasmid, C-GFPSpark tag||HG10298-ACG|
|Human MERTK / Mer ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10298-ACR|
|Human MERTK / Mer ORF mammalian expression plasmid, C-Flag tag||HG10298-CF|
|Human MERTK / Mer ORF mammalian expression plasmid, C-His tag||HG10298-CH|
|Human MERTK / Mer ORF mammalian expression plasmid, C-Myc tag||HG10298-CM|
|Human MERTK / Mer ORF mammalian expression plasmid, C-HA tag||HG10298-CY|
|Human MERTK / Mer Gene cDNA clone plasmid||HG10298-M|
|Human MERTK / Mer ORF mammalian expression plasmid, N-Flag tag||HG10298-NF|
|Human MERTK / Mer ORF mammalian expression plasmid, N-His tag||HG10298-NH|
|Human MERTK / Mer ORF mammalian expression plasmid, N-Myc tag||HG10298-NM|
|Human MERTK / Mer ORF mammalian expression plasmid, N-HA tag||HG10298-NY|
|Human MERTK / Mer natural ORF mammalian expression plasmid||HG10298-UT|
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&Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. MERTK is localized in membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver. MERTK regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. MERTK plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38.