|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SerpinD1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10295-ACG|
|Human SerpinD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10295-ACR|
|Human SerpinD1 ORF mammalian expression plasmid, C-Flag tag||HG10295-CF|
|Human SerpinD1 ORF mammalian expression plasmid, C-His tag||HG10295-CH|
|Human SerpinD1 ORF mammalian expression plasmid, C-Myc tag||HG10295-CM|
|Human SerpinD1 ORF mammalian expression plasmid, C-HA tag||HG10295-CY|
|Human SerpinD1 Gene cDNA clone plasmid||HG10295-M|
|Human SerpinD1 ORF mammalian expression plasmid, N-Flag tag||HG10295-NF|
|Human SerpinD1 ORF mammalian expression plasmid, N-His tag||HG10295-NH|
|Human SerpinD1 ORF mammalian expression plasmid, N-Myc tag||HG10295-NM|
|Human SerpinD1 ORF mammalian expression plasmid, N-HA tag||HG10295-NY|
|Human SerpinD1 natural ORF mammalian expression plasmid||HG10295-UT|
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SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.