|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CD64 ORF mammalian expression plasmid, C-GFPSpark tag||HG10256-ACG|
|Human CD64 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10256-ACR|
|Human CD64 ORF mammalian expression plasmid, C-Flag tag||HG10256-CF|
|Human CD64 ORF mammalian expression plasmid, C-His tag||HG10256-CH|
|Human CD64 ORF mammalian expression plasmid, C-Myc tag||HG10256-CM|
|Human CD64 ORF mammalian expression plasmid, C-HA tag||HG10256-CY|
|Human CD64 Gene cDNA clone plasmid||HG10256-M|
|Human CD64 ORF mammalian expression plasmid, N-Flag tag||HG10256-NF|
|Human CD64 ORF mammalian expression plasmid, N-His tag||HG10256-NH|
|Human CD64 ORF mammalian expression plasmid, N-Myc tag||HG10256-NM|
|Human CD64 ORF mammalian expression plasmid, N-HA tag||HG10256-NY|
|Human CD64 natural ORF mammalian expression plasmid||HG10256-UT|
|Learn more about expression Vectors|
Mouse high affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.