|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ACVR2B ORF mammalian expression plasmid, C-GFPSpark tag||HG10229-ACG|
|Human ACVR2B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10229-ACR|
|Human ACVR2B ORF mammalian expression plasmid, C-Flag tag||HG10229-CF|
|Human ACVR2B ORF mammalian expression plasmid, C-His tag||HG10229-CH|
|Human ACVR2B ORF mammalian expression plasmid, C-Myc tag||HG10229-CM|
|Human ACVR2B ORF mammalian expression plasmid, C-HA tag||HG10229-CY|
|Human ACVR2B Gene cDNA clone plasmid||HG10229-M|
|Human ACVR2B ORF mammalian expression plasmid, N-Flag tag||HG10229-NF|
|Human ACVR2B ORF mammalian expression plasmid, N-His tag||HG10229-NH|
|Human ACVR2B ORF mammalian expression plasmid, N-Myc tag||HG10229-NM|
|Human ACVR2B ORF mammalian expression plasmid, N-HA tag||HG10229-NY|
|Human ACVR2B natural ORF mammalian expression plasmid||HG10229-UT|
|Learn more about expression Vectors|
ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.