|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus ART4 ORF mammalian expression plasmid, C-GFPSpark tag||CG90272-ACG|
|Rhesus ART4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90272-ACR|
|Rhesus ART4 ORF mammalian expression plasmid, C-Flag tag||CG90272-CF|
|Rhesus ART4 ORF mammalian expression plasmid, C-His tag||CG90272-CH|
|Rhesus ART4 ORF mammalian expression plasmid, C-Myc tag||CG90272-CM|
|Rhesus ART4 ORF mammalian expression plasmid, C-HA tag||CG90272-CY|
|Rhesus ART4 Gene cDNA clone plasmid||CG90272-G|
|Rhesus ART4 ORF mammalian expression plasmid, N-Flag tag||CG90272-NF|
|Rhesus ART4 ORF mammalian expression plasmid, N-His tag||CG90272-NH|
|Rhesus ART4 ORF mammalian expression plasmid, N-Myc tag||CG90272-NM|
|Rhesus ART4 ORF mammalian expression plasmid, N-HA tag||CG90272-NY|
|Rhesus ART4 natural ORF mammalian expression plasmid||CG90272-UT|
|Learn more about expression Vectors|
ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.