|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human KLK-13 ORF mammalian expression plasmid, C-GFPSpark tag||HG10199-ACG|
|Human KLK-13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10199-ACR|
|Human KLK-13 ORF mammalian expression plasmid, C-Flag tag||HG10199-CF|
|Human KLK-13 ORF mammalian expression plasmid, C-His tag||HG10199-CH|
|Human KLK-13 ORF mammalian expression plasmid, C-Myc tag||HG10199-CM|
|Human KLK-13 ORF mammalian expression plasmid, C-HA tag||HG10199-CY|
|Human KLK-13 Gene cDNA clone plasmid||HG10199-M|
|Human KLK-13 ORF mammalian expression plasmid, Flag tag||HG10199-M-F|
|Human KLK-13 ORF mammalian expression plasmid, N-Flag tag||HG10199-NF|
|Human KLK-13 ORF mammalian expression plasmid, N-His tag||HG10199-NH|
|Human KLK-13 ORF mammalian expression plasmid, N-Myc tag||HG10199-NM|
|Human KLK-13 ORF mammalian expression plasmid, N-HA tag||HG10199-NY|
|Human KLK-13 natural ORF mammalian expression plasmid||HG10199-UT|
|Learn more about expression Vectors|
Human tissue kallikrein 13 (hK13), also known as KLK-L4 (kallikrein-like gene 4), is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers, and is regarded as a novel cancer biomarker. In addition, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.