|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ESAM ORF mammalian expression plasmid, C-GFPSpark tag||HG10187-ACG|
|Human ESAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10187-ACR|
|Human ESAM ORF mammalian expression plasmid, C-Flag tag||HG10187-CF|
|Human ESAM ORF mammalian expression plasmid, C-His tag||HG10187-CH|
|Human ESAM ORF mammalian expression plasmid, C-Myc tag||HG10187-CM|
|Human ESAM ORF mammalian expression plasmid, C-HA tag||HG10187-CY|
|Human ESAM Gene cDNA clone plasmid||HG10187-M|
|Human ESAM ORF mammalian expression plasmid, Flag tag||HG10187-M-F|
|Human ESAM ORF mammalian expression plasmid, N-Flag tag||HG10187-NF|
|Human ESAM ORF mammalian expression plasmid, N-His tag||HG10187-NH|
|Human ESAM ORF mammalian expression plasmid, N-Myc tag||HG10187-NM|
|Human ESAM ORF mammalian expression plasmid, N-HA tag||HG10187-NY|
|Human ESAM natural ORF mammalian expression plasmid||HG10187-UT|
|Learn more about expression Vectors|
Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. It is specifically expressed at endothelial tight junctions and on activated platelets. ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. Previous studies have indicated that ESAM regulates angiogenesis in the primary tumor growth and endothelial permeability. It suggest that ESAM has a redundant functional role in physiological angiogenesis but serves a unique and essential role in pathological angiogenic processes such as tumor growth.