|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human Dkk1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10170-ACG|
|Human Dkk1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10170-ACR|
|Human Dkk1 ORF mammalian expression plasmid, C-Flag tag||HG10170-CF|
|Human Dkk1 ORF mammalian expression plasmid, C-His tag||HG10170-CH|
|Human Dkk1 ORF mammalian expression plasmid, C-Myc tag||HG10170-CM|
|Human Dkk1 ORF mammalian expression plasmid, C-HA tag||HG10170-CY|
|Human Dkk1 Gene cDNA clone plasmid||HG10170-M|
|Human Dkk1 ORF mammalian expression plasmid, N-Flag tag||HG10170-NF|
|Human Dkk1 ORF mammalian expression plasmid, N-His tag||HG10170-NH|
|Human Dkk1 ORF mammalian expression plasmid, N-Myc tag||HG10170-NM|
|Human Dkk1 ORF mammalian expression plasmid, N-HA tag||HG10170-NY|
|Human Dkk1 natural ORF mammalian expression plasmid||HG10170-UT|
|Learn more about expression Vectors|
Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.