|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CystatinF ORF mammalian expression plasmid, C-GFPSpark tag||HG10167-ACG|
|Human CystatinF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10167-ACR|
|Human CystatinF ORF mammalian expression plasmid, C-Flag tag||HG10167-CF|
|Human CystatinF ORF mammalian expression plasmid, C-His tag||HG10167-CH|
|Human CystatinF ORF mammalian expression plasmid, C-Myc tag||HG10167-CM|
|Human CystatinF ORF mammalian expression plasmid, C-HA tag||HG10167-CY|
|Human CystatinF Gene cDNA clone plasmid||HG10167-M|
|Human CystatinF ORF mammalian expression plasmid, N-Flag tag||HG10167-NF|
|Human CystatinF ORF mammalian expression plasmid, N-His tag||HG10167-NH|
|Human CystatinF ORF mammalian expression plasmid, N-Myc tag||HG10167-NM|
|Human CystatinF ORF mammalian expression plasmid, N-HA tag||HG10167-NY|
|Human CystatinF natural ORF mammalian expression plasmid||HG10167-UT|
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The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.