|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human BMP-7 ORF mammalian expression plasmid, C-GFPSpark tag||HG10083-ACG|
|Human BMP-7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10083-ACR|
|Human BMP-7 ORF mammalian expression plasmid, C-Flag tag||HG10083-CF|
|Human BMP-7 ORF mammalian expression plasmid, C-His tag||HG10083-CH|
|Human BMP-7 ORF mammalian expression plasmid, C-Myc tag||HG10083-CM|
|Human BMP-7 ORF mammalian expression plasmid, C-HA tag||HG10083-CY|
|Human BMP-7 Gene cDNA clone plasmid||HG10083-M|
|Human BMP-7 natural ORF mammalian expression plasmid, HA tag||HG10083-M-Y|
|Human BMP-7 ORF mammalian expression plasmid, N-Flag tag||HG10083-NF|
|Human BMP-7 ORF mammalian expression plasmid, N-His tag||HG10083-NH|
|Human BMP-7 ORF mammalian expression plasmid, N-Myc tag||HG10083-NM|
|Human BMP-7 ORF mammalian expression plasmid, N-HA tag||HG10083-NY|
|Human BMP-7 natural ORF mammalian expression plasmid||HG10083-UT|
|Learn more about expression Vectors|
BMP-7 (Bone morphogenetic protein 7), also known as osteogenic protein 1 (OP-1), is a bone morphogenetic protein which belongs to the TGF-β superfamily. OP-1 is expressed in the brain, kidneys and bladder. BMP-7 may be involved in bone homeostasis. Osteogenic protein 1 plays a key role in the transformation of mesenchymal cells into bone and cartilage. The phosphorylation of SMAD1 and SMAD5 can be induced by BMP-7, which in turn induce transcription of numerous osteogenic genes. BMP-7 treatment can also induce all of the genetic markers of osteoblast differentiation in many cell types. The expression of BMP-7 causes ventral phenotypes while its complete inhibition creates a dorsal phenotype. Human recombinant BMP-7 protein can be used to aid in the fusion of vertebral bodies to prevent neurologic trauma. It also functions in the treatment of tibial non-union, frequently in cases where a bone graft has failed. It is found that BMP7 has the potential for treatment of chronic kidney disease.