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Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-tagged

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CA8cDNA Clone Product Information
Gene_bank_ref_id:NM_004056.4
cDNA Size:873
cDNA Description:ORF Clone of Homo sapiens carbonic anhydrase VIII DNA.
Gene Synonym:CA8, CALS, CARP, CA-VIII
Species:Human
Vector:pCMV3-C-Myc
Restriction Site:
Tag Sequence:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-C-Myc Vector Information
 
Vector Name pCMV3-C-Myc
Vector Size 6164bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag Myc
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-C-Myc Physical Map

Schematic of pCMV3-C-Myc Multiple Cloning Sites

Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-tagged on other vectors
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10063-ACG$325
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10063-ACR$325
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10063-ANG$325
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10063-ANR$325
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10063-CF$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10063-CH$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10063-CM$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10063-CY$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone)HG10063-M$95
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedHG10063-M-F$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10063-M-N$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10063-NF$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10063-NH$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10063-NM$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10063-NY$295
Human CA VIII Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10063-UT$295
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Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
  • Size / Price
    Catalog:HG10063-CM
    List Price: $295.00  (Save $0.00)
    Price:$295.00      [How to order]
    Availability2-3 weeks
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