|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus LTBR ORF mammalian expression plasmid, C-GFPSpark tag||CG90101-ACG|
|Rhesus LTBR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90101-ACR|
|Rhesus LTBR ORF mammalian expression plasmid, C-Flag tag||CG90101-CF|
|Rhesus LTBR ORF mammalian expression plasmid, C-His tag||CG90101-CH|
|Rhesus LTBR ORF mammalian expression plasmid, C-Myc tag||CG90101-CM|
|Rhesus LTBR ORF mammalian expression plasmid, C-HA tag||CG90101-CY|
|Rhesus LTBR Gene cDNA clone plasmid||CG90101-G|
|Rhesus LTBR ORF mammalian expression plasmid, N-Flag tag||CG90101-NF|
|Rhesus LTBR ORF mammalian expression plasmid, N-His tag||CG90101-NH|
|Rhesus LTBR ORF mammalian expression plasmid, N-Myc tag||CG90101-NM|
|Rhesus LTBR ORF mammalian expression plasmid, N-HA tag||CG90101-NY|
|Rhesus LTBR natural ORF mammalian expression plasmid||CG90101-UT|
|Learn more about expression Vectors|
LTBR (lymphotoxin beta receptor (TNFR superfamily, member 3)) is a member of the tumor necrosis factor (TNF) family of receptors. Tumor necrosis factor receptor is a trimeric cytokine receptor that binds tumor necrosis factors. The receptor cooperates with an adaptor protein (such as TRADD, TRAF, RIP), which is important in determining the outcome of the response. LTBR is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. LTBR specifically binds the lymphotoxin membrane form (a complex of lymphotoxin-alpha and lymphtoxin-beta). LTBR and its ligand play a role in the development and organization of lymphoid tissue and tranformed cells. Activation of this protein can trigger apoptosis. Not only does the LTBR help trigger apoptosis, it can lead to the release of the cytokine interleukin 8. Overexpression of LTBR in HEK293 cells increases IL-8 promoter activity and leads to IL-8 release. It is also essential for development and organization of the secondary lymphoid organs and chemokine release.