|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus HFE2 ORF mammalian expression plasmid, C-GFPSpark tag||CG90061-ACG|
|Rhesus HFE2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90061-ACR|
|Rhesus HFE2 ORF mammalian expression plasmid, C-Flag tag||CG90061-CF|
|Rhesus HFE2 ORF mammalian expression plasmid, C-His tag||CG90061-CH|
|Rhesus HFE2 ORF mammalian expression plasmid, C-Myc tag||CG90061-CM|
|Rhesus HFE2 ORF mammalian expression plasmid, C-HA tag||CG90061-CY|
|Rhesus HFE2 Gene cDNA clone plasmid||CG90061-G|
|Rhesus HFE2 ORF mammalian expression plasmid, N-Flag tag||CG90061-NF|
|Rhesus HFE2 ORF mammalian expression plasmid, N-His tag||CG90061-NH|
|Rhesus HFE2 ORF mammalian expression plasmid, N-Myc tag||CG90061-NM|
|Rhesus HFE2 ORF mammalian expression plasmid, N-HA tag||CG90061-NY|
|Rhesus HFE2 natural ORF mammalian expression plasmid||CG90061-UT|
|Learn more about expression Vectors|
Hemojuvelin, also known as HFE2, is a membrane-bound and soluble protein which belongs to the repulsive guidance molecule (RGM) family. It is known that RGMs function through Neogenin, a homologue of the Netrin receptor deleted in colon cancer. In mammals, RGM family consists of three glycoproteins which have discrete expression patterns and functions (RGM-A, RGM-B, and RGM-C). Hemojuvelin is expressed in adult and fetal liver, heart, and skeletal muscle. Hemojuvelin acts as a bone morphogenetic protein (BMP) coreceptor. Enhancement of BMP signaling regulates hepcidin (HAMP) expression and iron metabolism. It plays a key role in iron metabolism. Hemojuvelin represents the cellular receptor for hepcidin. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. Defects in hemojuvelin are the cause of hemochromatosis type 2A, also known as juvenile hemochromatosis (JH).