|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus CD3E ORF mammalian expression plasmid, C-GFPSpark tag||CG90047-ACG|
|Rhesus CD3E ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90047-ACR|
|Rhesus CD3E ORF mammalian expression plasmid, C-Flag tag||CG90047-CF|
|Rhesus CD3E ORF mammalian expression plasmid, C-His tag||CG90047-CH|
|Rhesus CD3E ORF mammalian expression plasmid, C-Myc tag||CG90047-CM|
|Rhesus CD3E ORF mammalian expression plasmid, C-HA tag||CG90047-CY|
|Rhesus CD3E Gene cDNA clone plasmid||CG90047-G|
|Rhesus CD3E ORF mammalian expression plasmid, N-Flag tag||CG90047-NF|
|Rhesus CD3E ORF mammalian expression plasmid, N-His tag||CG90047-NH|
|Rhesus CD3E ORF mammalian expression plasmid, N-Myc tag||CG90047-NM|
|Rhesus CD3E ORF mammalian expression plasmid, N-HA tag||CG90047-NY|
|Rhesus CD3E natural ORF mammalian expression plasmid||CG90047-UT|
|Learn more about expression Vectors|
T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.