|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Ferret SERPING1 ORF mammalian expression plasmid, C-GFPSpark tag||FG60083-ACG|
|Ferret SERPING1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||FG60083-ACR|
|Ferret SERPING1 ORF mammalian expression plasmid, C-Flag tag||FG60083-CF|
|Ferret SERPING1 ORF mammalian expression plasmid, C-His tag||FG60083-CH|
|Ferret SERPING1 ORF mammalian expression plasmid, C-Myc tag||FG60083-CM|
|Ferret SERPING1 ORF mammalian expression plasmid, C-HA tag||FG60083-CY|
|Ferret SERPING1 Gene cDNA clone plasmid||FG60083-G|
|Ferret SERPING1 ORF mammalian expression plasmid, N-Flag tag||FG60083-NF|
|Ferret SERPING1 ORF mammalian expression plasmid, N-His tag||FG60083-NH|
|Ferret SERPING1 ORF mammalian expression plasmid, N-Myc tag||FG60083-NM|
|Ferret SERPING1 ORF mammalian expression plasmid, N-HA tag||FG60083-NY|
|Ferret SERPING1 natural ORF mammalian expression plasmid||FG60083-UT|
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Plasma protease C1 inhibitor, also known as C1-inhibiting factor, C1-INH, C1 esterase inhibitor, SERPING1 and C1IN, is a serine proteinase inhibitor (serpin) that regulates activation of both the complement and contact systems. By its C-terminal part (serpin domain), characterized by three beta-sheets and an exposed mobile reactive loop, C1-INH binds, and blocks the activity of its target proteases. The N-terminal end (nonserpin domain) confers to C1-INH the capacity to bind lipopolysaccharides and E-selectin. Owing to this moiety, C1-INH intervenes in regulation of the inflammatory reaction. The heterozygous deficiency of C1-INH results in hereditary angioedema (HAE). Owing to its ability to modulate the contact and complement systems and the convincing safety profile, plasma-derived C1 inhibitor is an attractive therapeutic protein to treat inflammatory diseases other than HAE. Deficiency of C1 inhibitor results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa. C1 inhibitor may prove useful in a variety of other diseases including septic shock, reperfusion injury, hyperacute transplant rejection, traumatic and hemorrhagic shock, and the increased vascular permeability associated with thermal injury, interleukin-2 therapy and cardiopulmonary bypass.