|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine GM2A ORF mammalian expression plasmid, C-GFPSpark tag||DG70177-ACG|
|Canine GM2A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70177-ACR|
|Canine GM2A ORF mammalian expression plasmid, C-Flag tag||DG70177-CF|
|Canine GM2A ORF mammalian expression plasmid, C-His tag||DG70177-CH|
|Canine GM2A ORF mammalian expression plasmid, C-Myc tag||DG70177-CM|
|Canine GM2A ORF mammalian expression plasmid, C-HA tag||DG70177-CY|
|Canine GM2A ORF mammalian expression plasmid, N-Flag tag||DG70177-NF|
|Canine GM2A ORF mammalian expression plasmid, N-His tag||DG70177-NH|
|Canine GM2A ORF mammalian expression plasmid, N-Myc tag||DG70177-NM|
|Canine GM2A ORF mammalian expression plasmid, N-HA tag||DG70177-NY|
|Canine GM2A Gene cDNA clone plasmid||DG70177-U|
|Canine GM2A natural ORF mammalian expression plasmid||DG70177-UT|
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GM2A (GM2 ganglioside activator), is a lipid transfer protein which belongs to the ML domain family. GM2A can accommodate several single chain phospholipids and fatty acids. It also exhibits some calcium-independent phospholipase activity. GM2A binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. GM2A acts as a substrate specific co-factor for the lysosomal enzyme β-hexosaminidase A. β-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. Defects in GM2A are the cause of GM2-gangliosidosis type AB (GM2GAB), also known as Tay-Sachs disease AB variant.