|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine TNFRSF19 ORF mammalian expression plasmid, C-GFPSpark tag||DG70094-ACG|
|Canine TNFRSF19 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70094-ACR|
|Canine TNFRSF19 ORF mammalian expression plasmid, C-Flag tag||DG70094-CF|
|Canine TNFRSF19 ORF mammalian expression plasmid, C-His tag||DG70094-CH|
|Canine TNFRSF19 ORF mammalian expression plasmid, C-Myc tag||DG70094-CM|
|Canine TNFRSF19 ORF mammalian expression plasmid, C-HA tag||DG70094-CY|
|Canine TNFRSF19 Gene cDNA clone plasmid||DG70094-G|
|Canine TNFRSF19 ORF mammalian expression plasmid, N-Flag tag||DG70094-NF|
|Canine TNFRSF19 ORF mammalian expression plasmid, N-His tag||DG70094-NH|
|Canine TNFRSF19 ORF mammalian expression plasmid, N-Myc tag||DG70094-NM|
|Canine TNFRSF19 ORF mammalian expression plasmid, N-HA tag||DG70094-NY|
|Canine TNFRSF19 natural ORF mammalian expression plasmid||DG70094-UT|
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Tumor necrosis factor receptor superfamily, member 19 (TNFRSF19), also known as TAJ-alpha or TROY, is a member of the TNF-receptor superfamily. TNFRSF19/TROY expression is detected in the pulmonary epithelium and the ductal epithelium of the prostate and parotid glands. TNFRSF19/TROY expression is detected in some adenocarcinoma cell lines that arise from this tissue. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. TNFRSF19/TROY is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. TNFRSF19/TROY was negatively regulated by adipogenic transcription factor CCAAT/enhancer-binding proteins (C/EBP). TNFRSF19 signals activation of the Jnk pathway and induces cell death. Overexpression of TNFRSF19 also signals NFB activation, comparable and similar to that by p75NGFR. TNFRSF19/TROY is capable of activating key signaling pathways of the TNF receptor family, and its predominant expression patterns suggest that it plays a role in the growth and regulation of epithelial tissues.