|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine IL22RA1 ORF mammalian expression plasmid, C-GFPSpark tag||DG70083-ACG|
|Canine IL22RA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70083-ACR|
|Canine IL22RA1 ORF mammalian expression plasmid, C-Flag tag||DG70083-CF|
|Canine IL22RA1 ORF mammalian expression plasmid, C-His tag||DG70083-CH|
|Canine IL22RA1 ORF mammalian expression plasmid, C-Myc tag||DG70083-CM|
|Canine IL22RA1 ORF mammalian expression plasmid, C-HA tag||DG70083-CY|
|Canine IL22RA1 Gene cDNA clone plasmid||DG70083-G|
|Canine IL22RA1 ORF mammalian expression plasmid, N-Flag tag||DG70083-NF|
|Canine IL22RA1 ORF mammalian expression plasmid, N-His tag||DG70083-NH|
|Canine IL22RA1 ORF mammalian expression plasmid, N-Myc tag||DG70083-NM|
|Canine IL22RA1 ORF mammalian expression plasmid, N-HA tag||DG70083-NY|
|Canine IL22RA1 natural ORF mammalian expression plasmid||DG70083-UT|
|Learn more about expression Vectors|
IL-22R belongs to the type II cytokine receptor family. It contains 2 fibronectin type-III domains and is expressed in colon, liver, lung, pancreas and kidney. IL-22R also can be expressed in keratinocytes of normal skin as well as in psoriatic skin lesion. Overexpression of IL-22R can be detected in synovial fluid cells from rheumatoid arthritis and spondyloarthropathy patients. IL-22R is a component of the receptor for IL20, IL22 and IL24. The component of IL-22R formed by IL22RA1 and IL10RB enables IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. Component of one of the receptor for IL20 and IL24 formed by IL22RA1 and IL20RB also signaling through STATs activation. IL-22R mediates IL24 antiangiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation.