|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine IL2RB ORF mammalian expression plasmid, C-GFPSpark tag||DG70082-ACG|
|Canine IL2RB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70082-ACR|
|Canine IL2RB ORF mammalian expression plasmid, C-Flag tag||DG70082-CF|
|Canine IL2RB ORF mammalian expression plasmid, C-His tag||DG70082-CH|
|Canine IL2RB ORF mammalian expression plasmid, C-Myc tag||DG70082-CM|
|Canine IL2RB ORF mammalian expression plasmid, C-HA tag||DG70082-CY|
|Canine IL2RB Gene cDNA clone plasmid||DG70082-G|
|Canine IL2RB ORF mammalian expression plasmid, N-Flag tag||DG70082-NF|
|Canine IL2RB ORF mammalian expression plasmid, N-His tag||DG70082-NH|
|Canine IL2RB ORF mammalian expression plasmid, N-Myc tag||DG70082-NM|
|Canine IL2RB ORF mammalian expression plasmid, N-HA tag||DG70082-NY|
|Canine IL2RB natural ORF mammalian expression plasmid||DG70082-UT|
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Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.