|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine EFNA5 ORF mammalian expression plasmid, C-GFPSpark tag||DG70072-ACG|
|Canine EFNA5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70072-ACR|
|Canine EFNA5 ORF mammalian expression plasmid, C-Flag tag||DG70072-CF|
|Canine EFNA5 ORF mammalian expression plasmid, C-His tag||DG70072-CH|
|Canine EFNA5 ORF mammalian expression plasmid, C-Myc tag||DG70072-CM|
|Canine EFNA5 ORF mammalian expression plasmid, C-HA tag||DG70072-CY|
|Canine EFNA5 Gene cDNA clone plasmid||DG70072-G|
|Canine EFNA5 ORF mammalian expression plasmid, N-Flag tag||DG70072-NF|
|Canine EFNA5 ORF mammalian expression plasmid, N-His tag||DG70072-NH|
|Canine EFNA5 ORF mammalian expression plasmid, N-Myc tag||DG70072-NM|
|Canine EFNA5 ORF mammalian expression plasmid, N-HA tag||DG70072-NY|
|Canine EFNA5 natural ORF mammalian expression plasmid||DG70072-UT|
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Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.