|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine ACVRL1 ORF mammalian expression plasmid, C-GFPSpark tag||DG70049-ACG|
|Canine ACVRL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70049-ACR|
|Canine ACVRL1 ORF mammalian expression plasmid, C-Flag tag||DG70049-CF|
|Canine ACVRL1 ORF mammalian expression plasmid, C-His tag||DG70049-CH|
|Canine ACVRL1 ORF mammalian expression plasmid, C-Myc tag||DG70049-CM|
|Canine ACVRL1 ORF mammalian expression plasmid, C-HA tag||DG70049-CY|
|Canine ACVRL1 Gene cDNA clone plasmid||DG70049-G|
|Canine ACVRL1 ORF mammalian expression plasmid, N-Flag tag||DG70049-NF|
|Canine ACVRL1 ORF mammalian expression plasmid, N-His tag||DG70049-NH|
|Canine ACVRL1 ORF mammalian expression plasmid, N-Myc tag||DG70049-NM|
|Canine ACVRL1 ORF mammalian expression plasmid, N-HA tag||DG70049-NY|
|Canine ACVRL1 natural ORF mammalian expression plasmid||DG70049-UT|
|Learn more about expression Vectors|
ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP).