|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine FGF1 ORF mammalian expression plasmid, C-Flag tag||DG70039-CF|
|Canine FGF1 ORF mammalian expression plasmid, C-His tag||DG70039-CH|
|Canine FGF1 ORF mammalian expression plasmid, C-Myc tag||DG70039-CM|
|Canine FGF1 ORF mammalian expression plasmid, C-HA tag||DG70039-CY|
|Canine FGF1 ORF mammalian expression plasmid, N-Flag tag||DG70039-NF|
|Canine FGF1 ORF mammalian expression plasmid, N-His tag||DG70039-NH|
|Canine FGF1 ORF mammalian expression plasmid, N-Myc tag||DG70039-NM|
|Canine FGF1 ORF mammalian expression plasmid, N-HA tag||DG70039-NY|
|Canine FGF1 natural ORF mammalian expression plasmid||DG70039-UT|
|Learn more about expression Vectors|
aFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1–4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.