|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine ANGPT2 ORF mammalian expression plasmid, C-GFPSpark tag||DG70034-ACG|
|Canine ANGPT2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70034-ACR|
|Canine ANGPT2 ORF mammalian expression plasmid, C-Flag tag||DG70034-CF|
|Canine ANGPT2 ORF mammalian expression plasmid, C-His tag||DG70034-CH|
|Canine ANGPT2 ORF mammalian expression plasmid, C-Myc tag||DG70034-CM|
|Canine ANGPT2 ORF mammalian expression plasmid, C-HA tag||DG70034-CY|
|Canine ANGPT2 Gene cDNA clone plasmid||DG70034-G|
|Canine ANGPT2 ORF mammalian expression plasmid, N-Flag tag||DG70034-NF|
|Canine ANGPT2 ORF mammalian expression plasmid, N-His tag||DG70034-NH|
|Canine ANGPT2 ORF mammalian expression plasmid, N-Myc tag||DG70034-NM|
|Canine ANGPT2 ORF mammalian expression plasmid, N-HA tag||DG70034-NY|
|Canine ANGPT2 natural ORF mammalian expression plasmid||DG70034-UT|
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Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.