|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine BDNF ORF mammalian expression plasmid, C-GFPSpark tag||DG70029-ACG|
|Canine BDNF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70029-ACR|
|Canine BDNF ORF mammalian expression plasmid, C-Flag tag||DG70029-CF|
|Canine BDNF ORF mammalian expression plasmid, C-His tag||DG70029-CH|
|Canine BDNF ORF mammalian expression plasmid, C-Myc tag||DG70029-CM|
|Canine BDNF ORF mammalian expression plasmid, C-HA tag||DG70029-CY|
|Canine BDNF Gene cDNA clone plasmid||DG70029-G|
|Canine BDNF ORF mammalian expression plasmid, N-Flag tag||DG70029-NF|
|Canine BDNF ORF mammalian expression plasmid, N-His tag||DG70029-NH|
|Canine BDNF ORF mammalian expression plasmid, N-Myc tag||DG70029-NM|
|Canine BDNF ORF mammalian expression plasmid, N-HA tag||DG70029-NY|
|Canine BDNF natural ORF mammalian expression plasmid||DG70029-UT|
|Learn more about expression Vectors|
BDNF is a member of the nerve growth factor family. It is highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. It also can be detected in heart, lung, skeletal muscle, testis, prostate and placenta. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. During development, BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. It participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. It functions as the major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.