|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine NGF ORF mammalian expression plasmid, C-GFPSpark tag||DG70027-ACG|
|Canine NGF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70027-ACR|
|Canine NGF ORF mammalian expression plasmid, C-Flag tag||DG70027-CF|
|Canine NGF ORF mammalian expression plasmid, C-His tag||DG70027-CH|
|Canine NGF ORF mammalian expression plasmid, C-Myc tag||DG70027-CM|
|Canine NGF ORF mammalian expression plasmid, C-HA tag||DG70027-CY|
|Canine NGF Gene cDNA clone plasmid||DG70027-G|
|Canine NGF ORF mammalian expression plasmid, N-Flag tag||DG70027-NF|
|Canine NGF ORF mammalian expression plasmid, N-His tag||DG70027-NH|
|Canine NGF ORF mammalian expression plasmid, N-Myc tag||DG70027-NM|
|Canine NGF ORF mammalian expression plasmid, N-HA tag||DG70027-NY|
|Canine NGF natural ORF mammalian expression plasmid||DG70027-UT|
|Learn more about expression Vectors|
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.